منابع مشابه
Histones bundle F-actin filaments and affect actin structure
Histones are small polycationic proteins complexed with DNA located in the cell nucleus. Upon apoptosis they are secreted from the cells and react with extracellular polyanionic compounds. Actin which is a polyanionic protein, is also secreted from necrotic cells and interacts with histones. We showed that both histone mixture (histone type III) and the recombinant H2A histone bundles F-actin, ...
متن کاملActin-binding proteins sensitively mediate F-actin bundle stiffness.
Bundles of filamentous actin (F-actin) form primary structural components of a broad range of cytoskeletal processes including filopodia, sensory hair cell bristles and microvilli. Actin-binding proteins (ABPs) allow the cell to tailor the dimensions and mechanical properties of the bundles to suit specific biological functions. Therefore, it is important to obtain quantitative knowledge on the...
متن کاملMeasuring Protein Binding to F-actin by Co-sedimentation
Filamentous actin (F-actin) organization within cells is regulated by a large number of actin-binding proteins that control actin nucleation, growth, cross-linking and/or disassembly. This protocol describes a technique - the actin co-sedimentation, or pelleting, assay - to determine whether a protein or protein domain binds F-actin and to measure the affinity of the interaction (i.e., the diss...
متن کاملThe polyelectrolyte nature of F-actin and the mechanism of actin bundle formation.
Polymerized (F-)actin is induced to form bundles by a number of polycations including divalent metal ions, Co(NH3)63+, and basic polypeptides. The general features of bundle formation are largely independent of the specific structure of the bundling agent used. A threshold concentration of polycation is required to form lateral aggregates of actin filaments. The threshold concentration varies s...
متن کاملChlamydia trachomatis Tarp harbors distinct G and F actin binding domains that bundle actin filaments.
All species of Chlamydia undergo a unique developmental cycle that transitions between extracellular and intracellular environments and requires the capacity to invade new cells for dissemination. A chlamydial protein called Tarp has been shown to nucleate actin in vitro and is implicated in bacterial entry into human cells. Colocalization studies of ectopically expressed enhanced green fluores...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2019
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.3419